RRAS2 Rabbit Polyclonal Antibody
cat.: HA500379
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2.1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RRAS2 aa 2-201.
Positive control: SKOV-3 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue.
Subcellular location: Cell membrane, Golgi apparatus membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:800
Uniprot #: SwissProt: P62070 Human | P62071 Mouse | Q5BJU0 Rat
Alternative names: C86394 Oncogene RRAS2 Oncogene TC21 Ras like protein TC21 Ras related protein R Ras2 Ras-like protein TC21 Ras-related protein R-Ras2 Related RAS viral (r ras) oncogene homolog 2 Related RAS viral oncogene homolog 2 RRAS 2 RRAS2 RRAS2_HUMAN TC 21 TC21 Teratocarcinoma oncogene Teratocarcinoma oncogene TC21
Images
HA500379_1.jpg Fig1: Western blot analysis of RRAS2 on different lysates with Rabbit anti-RRAS2 antibody (HA500379) at 1/1,000 dilution.

Lane 1: SKOV-3 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: PC-12 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500379) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500379_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RRAS2 antibody (HA500379) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500379) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.