RRAS2 Rabbit Polyclonal Antibody
cat.: HA500379
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RRAS2 aa 2-201. |
| Positive control: | SKOV-3 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:800 |
| Uniprot #: | SwissProt: P62070 Human | P62071 Mouse | Q5BJU0 Rat |
| Alternative names: | C86394 Oncogene RRAS2 Oncogene TC21 Ras like protein TC21 Ras related protein R Ras2 Ras-like protein TC21 Ras-related protein R-Ras2 Related RAS viral (r ras) oncogene homolog 2 Related RAS viral oncogene homolog 2 RRAS 2 RRAS2 RRAS2_HUMAN TC 21 TC21 Teratocarcinoma oncogene Teratocarcinoma oncogene TC21 |
Images
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Fig1:
Western blot analysis of RRAS2 on different lysates with Rabbit anti-RRAS2 antibody (HA500379) at 1/1,000 dilution. Lane 1: SKOV-3 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500379) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RRAS2 antibody (HA500379) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500379) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.