Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Rat, Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 8 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human GNG3 aa 25-75. |
Positive control: | Mouse brain tissue lysate, rat brain tissue lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: P63215 Human | P63216 Mouse | G3V8K2 Rat |
Alternative names: | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3 GNG3 GNGT3 |
Fig1:
Western blot analysis of GNG3 on different lysates with Rabbit anti-GNG3 antibody (HA500380) at 1/500 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 11 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500380) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |