Histone H2B Rabbit Polyclonal Antibody
cat.: HA500381
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.7ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Histone H2B. |
| Positive control: | MCF-7 cell lysate, Hela cell lysate, A549 cell lysate, 293T cell lysate, THP-1 cell lysate, K562 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, zebrafish tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, rat large intestine tissue, rat liver tissue, human breast tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:5,000 |
| Uniprot #: | SwissProt: O60814 Human | P06899 Human | P23527 Human | P33778 Human | P57053 Human | P58876 Human | P62807 Human | Q16778 Human | Q5QNW6 Human | Q8N257 Human | Q93079 Human | Q99877 Human | Q99879 Human | Q99880 Human | P10854 Mouse | Q64475 Mouse | Q00729 Rat |
| Alternative names: | GL105 H2B GL105 H2B histone family member O H2B histone family member S H2B.1 H2B.1 B H2B.b H2B.c H2B.d H2B.e H2B.f H2B.j H2B.q H2B/b H2B/c H2B/d H2B/e H2B/f H2B/j H2B/o H2B/q H2BFB H2BFC H2BFD H2BFE H2BFF H2BFJ H2BFO H2BFQ H2BFS H2BGL105 H2BQ HIRIP2 HIST1H2BB HIST1H2BD HIST1H2BH HIST1H2BL HIST1H2BM HIST1H2BN HIST2H2BE histone cluster 2 Histone H2B histone H2B GL105 Histone H2B type 1 B Histone H2B type 1 D Histone H2B type 1 H Histone H2B type 1 L Histone H2B type 1 M Histone H2B type 1 N Histone H2B type 2 E Histone H2B.q histone protein |
Images
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Fig1:
Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (HA500381) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: 293T cell lysate (10 µg/Lane) Lane 5: THP-1 cell lysate (10 µg/Lane) Lane 6: K562 cell lysate (10 µg/Lane) Lane 7: HL-60 cell lysate (10 µg/Lane) Lane 8: NIH/3T3 cell lysate (10 µg/Lane) Lane 9: Zebrafish tissue lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (10 µg/Lane) Lane 11: RAW264.7 cell lysate (10 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500381) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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