PPP2R1B Rabbit Polyclonal Antibody
cat.: HA500383
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.4ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPP2R1B aa 100-350. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, RAW264.7 cell lysate, 293 cell lysate, rat brain liver tissue lysate, rat liver tissue lysate, mouse brain tissue lysate, human pancreas tissue, SiHa. |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:600 1:200 |
| Uniprot #: | SwissProt: P30154 Human | Q7TNP2 Mouse | Q4QQT4 Rat |
| Alternative names: | 2AAB_HUMAN beta isoform of regulatory subunit A, protein phosphatase 2 PP2A A beta PP2A subunit A isoform PR65-beta PP2A subunit A isoform R1-beta PP2A, subunit A, PR65 beta isoform PP2A, subunit A, R1 beta isoform PPP2R1B PPP4R1 PR65B protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform Protein phosphatase 2, regulatory subunit A, beta Protein phosphatase 2, structural/regulatory subunit A, beta protein phosphatase 2, structural/regulatory subunit A, beta isoform serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform |
Images
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Fig1:
Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA500383) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: RAW264.7 cell lysate (10 µg/Lane) Lane 4: 293 cell lysate (10 µg/Lane) Lane 5: Rat brain liver tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500383) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PPP2R1B antibody (HA500383) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500383) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling PPP2R1B with Rabbit anti-PPP2R1B antibody (HA500383) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP2R1B antibody (HA500383) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.