TNFSF14 Rabbit Polyclonal Antibody
cat.: HA500384
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TNFSF14 aa 190-240. |
| Positive control: | Mouse liver tissue lysates, human liver tissue, human colon tissue, human kidney tissue. |
| Subcellular location: | Cell membrane, Secreted, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: O43557 Human | Q9QYH9 Mouse |
| Alternative names: | CD 258 CD258 CD258 antigen Delta transmembrane LIGHT Herpes virus entry mediator ligand Herpesvirus entry mediator A Herpesvirus entry mediator ligand herpesvirus entry mediator-ligand HVEM L HVEM-L HVEML Ligand for herpesvirus entry mediator LIGHT LTg soluble form TNF14 TNF14_HUMAN TNFSF 14 Tnfsf14 TNFSF14 protein TR 2 TR2 Tumor necrosis factor (ligand) superfamily, member 14 Tumor necrosis factor ligand superfamily member 14 Tumor necrosis factor ligand superfamily, member 14 Tumor necrosis factor receptor like 2 tumor necrosis factor receptor-like 2 Tumor necrosis factor superfamily member 14 Tumor necrosis factor superfamily member LIGHT |
Images
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Fig1:
Western blot analysis of TNFSF14 on mouse liver tissue lysates with Rabbit anti-TNFSF14 antibody (HA500384) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500384) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TNFSF14 antibody (HA500384) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500384) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TNFSF14 antibody (HA500384) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500384) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TNFSF14 antibody (HA500384) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500384) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.