EIF2S1 Rabbit Polyclonal Antibody
cat.: HA500385
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EIF2S1 aa 116-315/315. |
| Positive control: | HepG2 cell lysate, A431 cell lysate, Raji cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse colon tissue lysate, rat spleen tissue lysate, human kidney tissue, Hela, A375, NCI-H441. |
| Subcellular location: | Cytoplasm, Stress granule, cytosol, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell IP |
1:500-1,000 1:1,900 1:500-1:1,000 1:100-1:200 1-2μg/sample |
| Uniprot #: | SwissProt: P05198 Human | Q6ZWX6 Mouse | P68101 Rat |
| Alternative names: | EIF 2 alpha EIF 2 EIF 2A EIF 2alpha eIF-2-alpha eIF-2A EIF-2alpha EIF2 alpha EIF2 EIF2A EIF2S1 Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa Eukaryotic translation initiation factor 2 subunit 1 alpha Eukaryotic translation initiation factor 2 subunit 1 Eukaryotic translation initiation factor 2 subunit alpha IF2A_HUMAN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EIF2S1 antibody (HA500385) at 1/1,900 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500385) at 1/1,900 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Flow cytometric analysis of Hela cells labeling EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500385, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Immunocytochemistry analysis of A375 cells labeling EIF2S1 with Rabbit anti-EIF2S1 antibody (HA500385) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EIF2S1 antibody (HA500385) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of NCI-H441 cells labeling EIF2S1 with Rabbit anti-EIF2S1 antibody (HA500385) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EIF2S1 antibody (HA500385) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
EIF2S1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA500385 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA500385 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA500385 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA500385 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
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Fig6:
Western blot analysis of EIF2S1 on different lysates with Rabbit anti-EIF2S1 antibody (HA500385) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell)cell lysate Lane 2: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 3:NIH/3T3 (Mouse fibroblast) cell lysate Exposure time: 24 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500385, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36.1 kDa Observed band size: 36 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.