CD23 Rabbit Polyclonal Antibody
cat.: HA500386
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD23 aa 250-321 / 321 (Extracellular). |
| Positive control: | Human tonsil tissue, Raji. |
| Subcellular location: | Secreted, cell membrane. |
| Recommended Dilutions:
IHC-P FC |
1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P06734 Human |
| Alternative names: | Blast 2 BLAST-2 Blast2 C type lectin domain family 4 member J C-type lectin domain family 4 member J C-type lectin domain family 4, member J CD 23 CD 23A CD23 CD23 antigen CD23A CLEC 4J CLEC4J Fc epsilon receptor II Fc epsilon RII Fc fragment of IgE low affinity II receptor for Fc fragment of IgE receptor II Fc fragment of IgE, low affinity II, receptor for (CD23) Fc of IgE, low affinity II, receptor for (CD23) Fc receptor IgE low affinity II alpha polypeptide Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a Fc-epsilon-RII FCE 2 FCE2 FCER 2 Fcer2 FCER2_HUMAN FCER2A FceRII IgE binding factor IgE receptor lymphocyte IgE-binding factor IGEBF Immunoglobulin E binding factor Immunoglobulin E receptor, low affinity II Immunoglobulin E-binding factor Immunoglobulin epsilon chain LEUKOCYTE ANTIGEN CD23 Low Affinity IgE Receptor Low affinity immunoglobulin epsilon Fc receptor Low affinity immunogl...... |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD23 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500386, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Flow cytometric analysis of CD23 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500386, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.