IL23R Rabbit Polyclonal Antibody
cat.: HA500387
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.88ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL23R aa 440-490. |
| Positive control: | MCF-7 cell lysate, 293 cell lysate, SH-SY5Y. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:200 |
| Uniprot #: | SwissProt: Q5VWK5 Human |
| Alternative names: | IL 23R IL-23 receptor IL-23R IL23 Receptor Il23r IL23R_HUMAN Interleukin 23 receptor Interleukin-23 receptor |
Images
|
Fig1:
Western blot analysis of IL23R on different lysates with Rabbit anti-IL23R antibody (HA500387) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 72 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500387) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling IL23R with Rabbit anti-IL23R antibody (HA500387) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL23R antibody (HA500387) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.