SOX3 Rabbit Polyclonal Antibody
cat.: HA500388
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.3ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SOX3 aa 20-70. |
| Positive control: | MCF-7 cell lysate, A549 cell lysate, Hela cell lysate, human cerebellum tissue lysate, mouse cerebellum tissue lysate, rat cerebellum tissue lysate, human testis tissue, human colon tissue, human esophagus tissue, human fallopian tube tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-5,000 1:600 |
| Uniprot #: | SwissProt: P41225 Human | P53784 Mouse Entrez Gene: 679158 Rat |
| Alternative names: | GHDX MRGH PHP PHPX sox3 SOX3_HUMAN SOXB SRY (sex determining region Y)-box 3 SRY Box 3 SRY-related HMG-box gene 3 Transcription factor Sox-3 |
Images
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Fig1:
Western blot analysis of SOX3 on different lysates with Rabbit anti-SOX3 antibody (HA500388) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell)(15 µg/Lane) Lane 2: MCF7 (Human breast cancer cell)(15 µg/Lane) Lane 3: A549 (Human lung adenocarcinoma cell)(15 µg/Lane) Lane 4: Mouse cerebellum tissue lysate(30 µg/Lane) Lane 5: Rat cerebellum tissue lysate(30 µg/Lane) Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500388, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 38 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.