SOX3 Rabbit Polyclonal Antibody
cat.: HA500388
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2.3ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human SOX3 aa 20-70.
Positive control: MCF-7 cell lysate, A549 cell lysate, Hela cell lysate, human cerebellum tissue lysate, mouse cerebellum tissue lysate, rat cerebellum tissue lysate, human testis tissue, human colon tissue, human esophagus tissue, human fallopian tube tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:600
Uniprot #: SwissProt: P41225 Human | P53784 Mouse
Entrez Gene: 679158 Rat
Alternative names: GHDX MRGH PHP PHPX sox3 SOX3_HUMAN SOXB SRY (sex determining region Y)-box 3 SRY Box 3 SRY-related HMG-box gene 3 Transcription factor Sox-3
Images
HA500388_1.jpg Fig1: Western blot analysis of SOX3 on different lysates with Rabbit anti-SOX3 antibody (HA500388) at 1/1,000 dilution.

Lane 1: MCF-7 cell lysate (10 µg/Lane)
Lane 2: A549 cell lysate (10 µg/Lane)
Lane 3: Hela cell lysate (10 µg/Lane)
Lane 4: Human cerebellum tissue lysate (20 µg/Lane)
Lane 5: Mouse cerebellum tissue lysate (20 µg/Lane)
Lane 6: Rat cerebellum tissue lysate (20 µg/Lane)

Predicted band size: 45 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500388_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500388_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500388_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500388_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.