PSMD4 Rabbit Polyclonal Antibody
cat.: HA500390
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.6ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMD4 aa 200-377. |
| Positive control: | Jurkat cell lysate, K562 cell lysate, HepG2 cell lysate, PC-12 cell lysate, Hela cell lysate, rat brain tissue, human colon tissue, human kidney tissue. |
| Subcellular location: | Cytosol, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: P55036 Human | O35226 Mouse Entrez Gene: 83499 Rat |
| Alternative names: | 26S protease subunit S5a 26S proteasome non ATPase regulatory subunit 4 26S proteasome non-ATPase regulatory subunit 4 26S proteasome regulatory subunit rpn10 26S proteasome regulatory subunit S5A AF 1 AF AF1 Angiocidin Antisecretory factor 1 ASF DS5a MCB 1 MCB1 Multiubiquitin chain binding protein Multiubiquitin chain-binding protein OTTHUMP00000059963 Prosome macropain Proteasome (prosome macropain) 26S subunit non ATPase 4 Proteasome 19S S5A Proteasome 26S non ATPase subunit 4 Proteasome 26S subunit non ATPase 4 PSMD 4 Psmd4 PSMD4_HUMAN pUB R5 pUBR5 Rpn 10 Rpn10 RPN10 homolog S5A S5a/antisecretory factor protein |
Images
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Fig1:
Western blot analysis of PSMD4 on different lysates with Rabbit anti-PSMD4 antibody (HA500390) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K562 cell lysate Lane 3: HepG2 cell lysate Lane 4: PC-12 cell lysate Lane 5: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMD4 antibody (HA500390) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500390) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMD4 antibody (HA500390) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500390) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PSMD4 antibody (HA500390) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500390) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.