PLCB3 Rabbit Polyclonal Antibody
cat.: HA500391
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.06ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 139 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 2-251. |
| Positive control: | MCF-7 cell lysate, RAW264.7 cell lysate, SH-SY5Y cell lysate, N2A cell lysate, mouse cerebellum tissue lysate, rat cerebellum tissue, human small intestine tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: Q01970 Human | Q9NQ66 Human | P51432 Mouse | Q9Z1B3 Mouse | P10687 Rat | Q99JE6 Rat |
| Alternative names: | 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-3 Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta-3 Phospholipase C beta 3 Phospholipase C beta 3 (phosphatidylinositol specific) Phospholipase C-beta-3 PLC beta 3 PLC-beta-3 Plcb3 PLCB3_HUMAN |
Images
|
Fig1:
Western blot analysis of PLCB3 on different lysates with Rabbit anti-PLCB3 antibody (HA500391) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: SH-SY5Y cell lysate (10 µg/Lane) Lane 4: N2A cell lysate (10 µg/Lane) Lane 5: Mouse cerebellum tissue lysate (20 µg/Lane) Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.