PLCB3 Rabbit Polyclonal Antibody
cat.: HA500391
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.06ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 139 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PLCB3 aa 2-251. |
| Positive control: | MCF-7 cell lysate, RAW264.7 cell lysate, SH-SY5Y cell lysate, N2A cell lysate, Mouse cerebellum tissue lysate, human small intestine tissue, rat cerebellum tissue, HepG2, PC-12. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:600 1:100-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q01970 Human | P51432 Mouse | Q99JE6 Rat |
| Alternative names: | 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-3 Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta-3 Phospholipase C beta 3 Phospholipase C beta 3 (phosphatidylinositol specific) Phospholipase C-beta-3 PLC beta 3 PLC-beta-3 Plcb3 PLCB3_HUMAN |
Images
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Fig1:
Western blot analysis of PLCB3 on different lysates with Rabbit anti-PLCB3 antibody (HA500391) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: SH-SY5Y cell lysate (10 µg/Lane) Lane 4: N2A cell lysate (10 µg/Lane) Lane 5: Mouse cerebellum tissue lysate (20 µg/Lane) Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HepG2 cells labeling PLCB3 with Rabbit anti-PLCB3 antibody (HA500391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB3 antibody (HA500391) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of PC-12 cells labeling PLCB3 with Rabbit anti-PLCB3 antibody (HA500391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB3 antibody (HA500391) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Flow cytometric analysis of PC-12 cells labeling PLCB3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500391, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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