PSMB10 Rabbit Polyclonal Antibody
cat.: HA500393
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.86ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within huaman PSMB10 aa 40-273. |
| Positive control: | Raji cell lysate, Daudi cell lysate, mouse colon tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:2,000 1:200 |
| Uniprot #: | SwissProt: P40306 Human | O35955 Mouse |
| Alternative names: | beta2i FLJ00366 LMP10 Low molecular mass protein 10 Macropain subunit MECl 1 Macropain subunit MECl-1 MECL1 MGC1665 Multicatalytic endopeptidase complex subunit MECl 1 Multicatalytic endopeptidase complex subunit MECl-1 OTTHUMP00000174858 Proteasome (prosome macropain) subunit beta type 10 Proteasome beta 10 subunit Proteasome catalytic subunit 2i Proteasome MECl 1 Proteasome MECl-1 Proteasome subunit beta 2i Proteasome subunit beta 7i Proteasome subunit beta type 10 Proteasome subunit beta type-10 Proteasome subunit beta-2i Proteasome subunit MECL1 PSB10_HUMAN PSMB 10 Psmb10 |
Images
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Fig1:
Western blot analysis of PSMB10 on different lysates with Rabbit anti-PSMB10 antibody (HA500393) at 1/500 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 26 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500393) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PSMB10 antibody (HA500393) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500393) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of SH-SY5Y cells labeling PSMB10 with Rabbit anti-PSMB10 antibody (HA500393) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMB10 antibody (HA500393) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.