PSME2 Rabbit Polyclonal Antibody
cat.: HA500394
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.6ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 2-239. |
| Positive control: | PC-12 cell lysate, Hela cell lysate, HepG2 cell lysate, PC-3M cell lysate, MCF-7 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, human rectal carcinoma tissue, Hela, HepG2. |
| Subcellular location: | Cytosol, Extracellular region or secreted, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:800 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UL46 Human | P97372 Mouse | Q63798 Rat |
| Alternative names: | 11S regulator complex beta subunit 11S regulator complex subunit beta Activator of multicatalytic protease subunit 2 Cell migration inducing protein 22 MCP activator 31 kD subunit PA28 beta PA28b PA28beta Proteasome (prosome macropain) activator subunit 2 (PA28 beta) Proteasome (prosome macropain) activator subunit 2 Proteasome activator 28 beta Proteasome activator 28 subunit beta Proteasome activator complex subunit 2 Proteasome activator hPA28 subunit beta Proteasome activator subunit 2 PSME 2 PSME2 PSME2_HUMAN REG beta REG-beta REGbeta |
Images
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Fig1:
Western blot analysis of PSME2 on different lysates with Rabbit anti-PSME2 antibody (HA500394) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane) Lane 2: Hela cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: PC-3M cell lysate (20 µg/Lane) Lane 5: MCF-7 cell lysate (20 µg/Lane) Lane 6: Mouse thymus tissue lysate (40 µg/Lane) Lane 7: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 27 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500394) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human rectal carcinoma tissue with Rabbit anti-PSME2 antibody (HA500394) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500394) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of Hela cells labeling PSME2 with Rabbit anti-PSME2 antibody (HA500394) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSME2 antibody (HA500394) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of HepG2 cells labeling PSME2 with Rabbit anti-PSME2 antibody (HA500394) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSME2 antibody (HA500394) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Flow cytometric analysis of HepG2 cells labeling PSME2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500394, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.