| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RBBP5 aa 430-480. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, K562 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-7 cell lysate |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q15291 Human | Q8BX09 Mouse Entrez Gene: 304794 Rat |
| Alternative names: | RBBP 5 RBBP-5 RBBP5 RBBP5_HUMAN RBQ 3 RBQ3 Retinoblastoma binding protein 5 Retinoblastoma binding protein RBQ3 Retinoblastoma binding protein5 Retinoblastoma-binding protein 5 Retinoblastoma-binding protein RBQ-3 SWD1 SWD1, Set1c WD40 repeat protein, homolog |
|
Fig1:
Western blot analysis of RBBP5 on different lysates with Rabbit anti-RBBP5 antibody (HA500395) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 4: C2C12 (Mouse myoblast) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 6: COS-7 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 20 µg/Lane Exposure time: 20 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500395, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa |