RBBP5 Rabbit Polyclonal Antibody
cat.: HA500395
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2.1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 59 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RBBP5 aa 430-480.
Positive control: Hela cell lysate, MCF-7 cell lysate, K562 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-7 cell lysate
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q15291 Human | Q8BX09 Mouse
Entrez Gene: 304794 Rat
Alternative names: RBBP 5 RBBP-5 RBBP5 RBBP5_HUMAN RBQ 3 RBQ3 Retinoblastoma binding protein 5 Retinoblastoma binding protein RBQ3 Retinoblastoma binding protein5 Retinoblastoma-binding protein 5 Retinoblastoma-binding protein RBQ-3 SWD1 SWD1, Set1c WD40 repeat protein, homolog
Images
HA500395_1.jpg Fig1: Western blot analysis of RBBP5 on different lysates with Rabbit anti-RBBP5 antibody (HA500395) at 1/1,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: MCF7 (Human breast cancer cell) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 4: C2C12 (Mouse myoblast) cell lysate
Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 6: COS-7 (African green monkey kidney fibroblast) cell lysate

Lysates/proteins at 20 µg/Lane
Exposure time: 20 seconds ; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500395, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 59 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.