| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.98ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RRAS aa 1-218. |
| Positive control: | NIH/3T3 cell lysate, A549 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P10301 Human | P10833 Mouse |
| Alternative names: | Oncogene RRAS p23 R Ras Ras related Protein Ras related protein R Ras Ras-related protein R-Ras related RAS viral (r ras) oncogene homolog RRAS RRAS_HUMAN |
|
Fig1:
Western blot analysis of RRAS on different lysates with Rabbit anti-RRAS antibody (HA500397) at 1/1,000 dilution. Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate (20 µg/Lane) Lane 2: rat spleen tissue lysate (40 µg/Lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500397, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23 kDa Observed band size: 23 kDa |