PLCB2 Rabbit Polyclonal Antibody
cat.: HA500399
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.99ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 134 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PLCB2 aa 950-1185. |
| Positive control: | THP-1 (Human acute monoblastic leukemia cell) cell lysate, HL-60. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100 |
| Uniprot #: | SwissProt: Q00722 Human |
| Alternative names: | 1-phosphatidylinositol-4 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 5-bisphosphate phosphodiesterase beta-2 FLJ38135 Phosphoinositide phospholipase C-beta-2 Phospholipase C-beta-2 PLC-beta-2 Plcb2 PLCB2_HUMAN |
Images
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Fig1:
Western blot analysis of PLCB2 on THP-1 (Human acute monoblastic leukemia cell) cell lysate with Rabbit anti-PLCB2 antibody (HA500399) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 18 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500399, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 134 kDa Observed band size: 134 kDa |
|
Fig2:
Immunocytochemistry analysis of HL-60 cells labeling PLCB2 with Rabbit anti-PLCB2 antibody (HA500399) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB2 antibody (HA500399) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.