PLCB2 Rabbit Polyclonal Antibody
cat.: HA500399
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.99ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 134 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PLCB2 aa 950-1185. |
| Positive control: | HL-60 cell lysate, THP-1 cell lysate, HL-60. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: Q00722 Human |
| Alternative names: | 1-phosphatidylinositol-4 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 5-bisphosphate phosphodiesterase beta-2 FLJ38135 Phosphoinositide phospholipase C-beta-2 Phospholipase C-beta-2 PLC-beta-2 Plcb2 PLCB2_HUMAN |
Images
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Fig1:
Western blot analysis of PLCB2 on different lysates with Rabbit anti-PLCB2 antibody (HA500399) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 134 kDa Observed band size: 134 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500399) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HL-60 cells labeling PLCB2 with Rabbit anti-PLCB2 antibody (HA500399) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB2 antibody (HA500399) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.