TAB1 Rabbit Polyclonal Antibody
cat.: HA500400
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.99ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TAB1 aa 1-250. |
| Positive control: | 293T cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysates, rat large intestine tissue, human lung carcinoma tissue, human testis tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:800 |
| Uniprot #: | SwissProt: Q15750 Human | Q8CF89 Mouse |
| Alternative names: | 2310012M03Rik 3'-Tab1 MAP3K7IP 1 MAP3K7IP1 MGC57664 Mitogen activated protein kinase kinase kinase 7 interacting protein 1 Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 TAB 1 TAB1 TAB1_HUMAN TAK1 binding protein 1 TAK1-binding protein 1 TGF beta activated kinase 1 binding protein 1 TGF-beta activated kinase 1/MAP3K7 binding protein 1 TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 TGF-beta-activated kinase 1-binding protein 1 Transforming growth factor beta activated kinase binding protein 1 |
Images
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Fig1:
Western blot analysis of TAB1 on different lysates with Rabbit anti-TAB1 antibody (HA500400) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TAB1 on mouse brain tissue lysates with Rabbit anti-TAB1 antibody (HA500400) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of TAB1 on different lysates with Rabbit anti-TAB1 antibody (HA500400) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TAB1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500400) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-TAB1 antibody (HA500400) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500400) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-TAB1 antibody (HA500400) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500400) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TAB1 antibody (HA500400) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500400) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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