UBE2I Rabbit Polyclonal Antibody
cat.: HA500401
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.92ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human UBE2I aa 2-158. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, Hela cell lysate, Jurkat cell lysate, PC-12 cell lysates. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P63279 Human | P63281 Rat |
| Alternative names: | C358B7.1 p18 SUMO 1 protein ligase SUMO conjugating enzyme UBC9 SUMO-conjugating enzyme UBC9 SUMO-protein ligase SUMO1 protein ligase UBC9 UBC9_HUMAN UBCE9 Ube2i Ubiquitin carrier protein 9 Ubiquitin carrier protein Ubiquitin carrier protein I Ubiquitin conjugating enzyme 9 Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) Ubiquitin conjugating enzyme UbcE2A Ubiquitin like protein SUMO 1 conjugating enzyme Ubiquitin protein ligase E2I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I |
Images
|
Fig1:
Western blot analysis of UBE2I on different lysates with Rabbit anti-UBE2I antibody (HA500401) at 1/1,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500401, 1/5,000 in in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 18 kDa Observed band size: 18 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.