UBE2I Rabbit Polyclonal Antibody
cat.: HA500401
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.92ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human UBE2I aa 2-158. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, Hela cell lysate, Jurkat cell lysate, PC-12 cell lysates, rat large intestine tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:800 |
| Uniprot #: | SwissProt: P63279 Human | P63281 Rat |
| Alternative names: | C358B7.1 p18 SUMO 1 protein ligase SUMO conjugating enzyme UBC9 SUMO-conjugating enzyme UBC9 SUMO-protein ligase SUMO1 protein ligase UBC9 UBC9_HUMAN UBCE9 Ube2i Ubiquitin carrier protein 9 Ubiquitin carrier protein Ubiquitin carrier protein I Ubiquitin conjugating enzyme 9 Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) Ubiquitin conjugating enzyme UbcE2A Ubiquitin like protein SUMO 1 conjugating enzyme Ubiquitin protein ligase E2I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I |
Images
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Fig1:
Western blot analysis of UBE2I on different lysates with Rabbit anti-UBE2I antibody (HA500401) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: 293T cell lysate Lane 3: Hela cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500401) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of UBE2I on PC-12 cell lysates with Rabbit anti-UBE2I antibody (HA500401) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500401) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-UBE2I antibody (HA500401) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500401) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.