Phospho-ATR (T1989) Rabbit Polyclonal Antibody
cat.: HA500405
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.13ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 301 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide containing pThr1989 site Peptides for Immunizaiton 1984-1995aa. |
| Positive control: | Hela cell lysate, HeLa treated with 1.5mM hydroxyurea for 16 hours cell lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1: 1,000 |
| Uniprot #: | SwissProt: Q13535 Human |
| Alternative names: | Ataxia telangiectasia and Rad3 related Ataxia telangiectasia and Rad3-related protein ATR ATR_HUMAN FCTCS FRAP Related Protein 1 FRAP-related protein 1 FRP1 MEC1 MEC1 mitosis entry checkpoint 1 homolog Protein kinase ATR RAC3 Rad3 related protein SCKL SCKL1 Serine/threonine protein kinase ATR Serine/threonine-protein kinase ATR |
Images
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Fig1:
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA500405) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HeLa treated with 1.5mM hydroxyurea for 16 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 301 kDa Observed band size: 301 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500405) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.