FZD2 Rabbit Polyclonal Antibody
cat.: HA500406
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.22ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FZD2 aa 301-327 (Extracellular domain). |
| Positive control: | 293 cell lysate, HepG2 cell lysate, Hela cell lysate, mouse heart tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: Q14332 Human | Q9JIP6 Mouse |
| Alternative names: | Frizzled homolog 2 Frizzled-2 Frizzled2 Fz 2 Fz-2 Fz2 FZD 2 FZD2 FZD2_HUMAN FzE 2 FzE2 hFz 2 hFz2 Mfz10 |
Images
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Fig1:
Western blot analysis of FZD2 on different lysates with Rabbit anti-FZD2 antibody (HA500406) at 1/1,000 dilution. Lane 1: 293 cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 63.5 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500406) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-FZD2 antibody (HA500406) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500406) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.