PSMD8 Rabbit Polyclonal Antibody
cat.: HA500408
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.31ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMD8 aa 150-350. |
| Positive control: | A549 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Hela cell lysates, SiHa. |
| Subcellular location: | Cytosol. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:200 |
| Uniprot #: | SwissProt: P48556 Human | Q9CX56 Mouse | Q3B8P5 Rat |
| Alternative names: | 26S proteasome non ATPase regulatory subunit 8 26S proteasome non-ATPase regulatory subunit 8 26S proteasome regulatory subunit p31 26S proteasome regulatory subunit RPN12 26S proteasome regulatory subunit S14 AAV38494 HIP6 HYPF MGC1660 Nin1p P31 Proteasome (prosome macropain) 26S subunit non ATPase 8 PSMD8 PSMD8_HUMAN Rpn12 S14 |
Images
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Fig1:
Western blot analysis of PSMD8 on different lysates with Rabbit anti-PSMD8 antibody (HA500408) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 30 kDa Exposure time: 5 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500408) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PSMD8 on Hela cell lysates with Rabbit anti-PSMD8 antibody (HA500408) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500408) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of SiHa cells labeling PSMD8 with Rabbit anti-PSMD8 antibody (HA500408) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD8 antibody (HA500408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.