PPP2R5B Rabbit Polyclonal Antibody
cat.: HA500409
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPP2R5B aa 1-250. |
| Positive control: | Mouse cerebellum tissue lysate, human cerebellum tissue lysate, rat cerebellum tissue lysate, SH-SY5Y cell lysates, human colon tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:600 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q15173 Human | Q6PD28 Mouse | Q80W83 Rat |
| Alternative names: | 2A5B_HUMAN B56B Beta isoform of regulatory subunit B56 protein phosphatase 2A FLJ35411 PP2A B subunit B' beta isoform PP2A B subunit B56 beta isoform PP2A B subunit isoform B' beta PP2A B subunit isoform B''-beta PP2A B subunit isoform B56-beta PP2A B subunit isoform PR61-beta PP2A B subunit isoform R5-beta PP2A B subunit PR61 beta isoform PP2A B subunit R5 beta isoform PPP2R5B PR 61B PR61B Protein phosphatase 2 regulatory subunit B (B56) beta Protein phosphatase 2 regulatory subunit B (B56) beta isoform Protein phosphatase 2 regulatory subunit B' beta Protein phosphatase 2 regulatory subunit B' beta isoform Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform |
Images
|
Fig1:
Western blot analysis of PPP2R5B on different lysates with Rabbit anti-PPP2R5B antibody (HA500409) at 1/500 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Human cerebellum tissue lysate Lane 3: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500409) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PPP2R5B on SH-SY5Y cell lysates with Rabbit anti-PPP2R5B antibody (HA500409) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500409) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PPP2R5B antibody (HA500409) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500409) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of SH-SY5Y cells labeling PPP2R5B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500409, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.