PIAS4 Rabbit Polyclonal Antibody
cat.: HA500411
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.83ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PIAS4 180-250. |
| Positive control: | Mouse testis tissue lysate, rat kidney tissue lysate, human kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: Q8N2W9 Human | Q3UGQ2 Mouse |
| Alternative names: | E3 SUMO protein ligase PIAS4 E3 SUMO-protein ligase PIAS4 FLJ12419 MGC35296 PAIASgamma PIAS 4 PIAS gamma PIAS-gamma Pias4 PIAS4_HUMAN PIASG PIASgamma PIASy Protein inhibitor of activated STAT 4 Protein inhibitor of activated STAT protein 4 Protein inhibitor of activated STAT protein gamma Protein inhibitor of activated STAT protein PIASy Zinc finger MIZ type containing 6 ZMIZ 6 ZMIZ6 |
Images
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Fig1:
Western blot analysis of PIAS4 on different lysates with Rabbit anti-PIAS4 antibody (HA500411) at 1/1,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56.5 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500411) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PIAS4 antibody (HA500411) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500411) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.