Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2.03ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 39 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human WNT9B aa 100-357. |
Positive control: | Mouse kidney tissue lysate, human kidney tissue lysate. |
Subcellular location: | Extracellular region or secreted. |
Recommended Dilutions:
WB |
1:1,000-1:5,000 |
Uniprot #: | SwissProt: O14905 Human | O35468 Mouse Entrez Gene: 7484 Human | 22412 Mouse |
Alternative names: | MGC124412 Protein Wnt 9b precursor Protein Wnt-14b Protein Wnt-9b Wingless type MMTV integration site 9B Wingless type MMTV integration site family member 15 Wingless type MMTV integration site family member 9B Wnt 14b Wnt-15 Wnt14b Wnt15 Wnt9b wingless type MMTV integration site family member 9B |
Fig1:
Western blot analysis of WNT9B on different lysates with Rabbit anti-WNT9B antibody (HA500412) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500412) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |