WNT9B Rabbit Polyclonal Antibody
cat.: HA500412
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2.03ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Recombinant protein within human WNT9B aa 100-357.
Positive control: Mouse kidney tissue lysate, human kidney tissue lysate.
Subcellular location: Extracellular region or secreted.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: O14905 Human | O35468 Mouse
Entrez Gene: 7484 Human | 22412 Mouse
Alternative names: MGC124412 Protein Wnt 9b precursor Protein Wnt-14b Protein Wnt-9b Wingless type MMTV integration site 9B Wingless type MMTV integration site family member 15 Wingless type MMTV integration site family member 9B Wnt 14b Wnt-15 Wnt14b Wnt15 Wnt9b wingless type MMTV integration site family member 9B
Images
HA500412_1.jpg Fig1: Western blot analysis of WNT9B on different lysates with Rabbit anti-WNT9B antibody (HA500412) at 1/1,000 dilution.

Lane 1: Human kidney tissue lysate
Lane 2: Mouse kidney tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 42 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500412) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.