TBP-1 Rabbit Polyclonal Antibody
cat.: HA500413
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.7ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TBP-1 aa 1-250. |
| Positive control: | 293T cell lysate, Hela cell lysate, MCF-7 cell lysate, human ovarian tumor tissue, human pancreas tissue, 293T. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P17980 Human | O88685 Mouse |
| Alternative names: | 26S protease regulatory subunit 6A 26S proteasome AAA-ATPase subunit RPT5 Human immunodeficiency virus tat transactivator binding protein 1 MGC8487 Proteasome (prosome macropain) 26S subunit ATPase 3 Proteasome 26S ATPase subunit 3 Proteasome 26S subunit ATPase 3 Proteasome subunit P50 PRS6A_HUMAN PSMC 3 PSMC3 Tat binding protein 1 TAT-binding protein 1 TBP-1 TBP1 |
Images
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Fig1:
Western blot analysis of TBP-1 on different lysates with Rabbit anti-TBP-1 antibody (HA500413) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500413) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-TBP-1 antibody (HA500413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TBP-1 antibody (HA500413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of 293T cells labeling TBP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500413, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.