PSMD11 Rabbit Polyclonal Antibody
cat.: HA500414
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PSMD11 aa 200-500. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-3 cell lysate, Hela cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse testis tissue, NIH/3T3. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:400 1:200 |
| Uniprot #: | SwissProt: O00231 Human | Q8BG32 Mouse | F1LMZ8 Rat |
| Alternative names: | 26S proteasome non-ATPase regulatory subunit 11 26S proteasome regulatory subunit 9 26S proteasome regulatory subunit p44.5 26S proteasome regulatory subunit RPN6 26S proteasome regulatory subunit S9 MGC3844 p44.5 protease 26S, subunit, 9 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 proteasome 26S subunit, non-ATPase, 11 PSD11_HUMAN PSMD 11 PSMD11 Rpn6 S9 |
Images
|
Fig1:
Western blot analysis of PSMD11 on different lysates with Rabbit anti-PSMD11 antibody (HA500414) at 1/2,000 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: PC-3 cell lysate (10 µg/Lane) Lane 6: Hela cell lysate (10 µg/Lane) Lane 7: Mouse testis tissue lysate (20 µg/Lane) Lane 8: Rat testis tissue lysate (20 µg/Lane) Lane 9: Mouse brain tissue lysate (20 µg/Lane) Lane 10: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 47 kDa Observed band size: 47/100 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500414) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PSMD11 antibody (HA500414) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500414) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling PSMD11 with Rabbit anti-PSMD11 antibody (HA500414) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD11 antibody (HA500414) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.