IRS-2 Rabbit Polyclonal Antibody
cat.: HA500415
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.07ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 137 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 1-250. |
| Positive control: | 293 cell lysate, SH-SY5Y cell lysate, A375 cell lysate, Hela cell lysate, PC-12 cell lysate, rat skeletal muscle tissue, human small intestine tissue. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600 |
| Uniprot #: | SwissProt: Q9Y4H2 Human | P81122 Mouse Entrez Gene: 29376 Rat |
| Alternative names: | Insulin receptor substrate 2 IRS 2 IRS-2 Irs2 IRS2_HUMAN |
Images
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Fig1:
Western blot analysis of IRS-2 on different lysates with Rabbit anti-IRS-2 antibody (HA500415) at 1/1,000 dilution. Lane 1: 293 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: A375 cell lysate Lane 4: Hela cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 137 kDa Observed band size: 150/200 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500415) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-IRS-2 antibody (HA500415) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500415) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-IRS-2 antibody (HA500415) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500415) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.