RYK Rabbit Polyclonal Antibody
cat.: HA500416
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.777ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RYK aa 300-550. |
| Positive control: | SH-SY5Y cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, MCF-7 cell lysates, NIH/3T3, SH-SY5Y. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:10,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P34925 Human | Q01887 Mouse Entrez Gene: 140585 Rat |
| Alternative names: | D3S3195 ERK 3 Hydroxyaryl protein kinase JTK 5 JTK 5A JTK5 JTK5A JTK5A protein tyrosine kinase receptor like tyrosine kinase receptor like tyrosine kinase precursor RYK 1 Ryk RYK_HUMAN RYK1 Tyrosine protein kinase RYK Tyrosine protein kinase RYK precursor Tyrosine-protein kinase RYK Vik |
Images
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Fig1:
Western blot analysis of RYK on different lysates with Rabbit anti-RYK antibody (HA500416) at 1/500 dilution. Lane 1: SH-SY5Y cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 68 kDa Observed band size: 52 kDa (C terminal fragment) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500416) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RYK on MCF-7 cell lysates with Rabbit anti-RYK antibody (HA500416) at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 42 kDa (intracellular domain) Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500416) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling RYK with Rabbit anti-RYK antibody (HA500416) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RYK antibody (HA500416) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of NIH/3T3 cells labeling RYK. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500416, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of SH-SY5Y cells labeling RYK. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500416, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.