KMT2D Rabbit Polyclonal Antibody
cat.: HA500417
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.047ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 593 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C terminal human KMT2D. |
| Positive control: | A549. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:500-2,000 1:100 |
| Uniprot #: | SwissProt: O14686 Human |
| Alternative names: | AAD10 ALL1 related gene ALL1-related protein ALR CAGL114 Histone-lysine N-methyltransferase MLL2 KABUK1 Kabuki make up syndrome Kabuki mental retardation syndrome KMS KMT2D Lysine N methyltransferase 2D MLL2 MLL2_HUMAN MLL4 Myeloid/lymphoid or mixed lineage leukemia 2 Myeloid/lymphoid or mixed-lineage leukemia protein 2 TNRC21 Trinucleotide repeat containing 21 |
Images
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Fig1:
Western blot analysis of KMT2D on recombinant protein with Rabbit anti-KMT2D antibody (HA500417) at 1/10,000 dilution. Lysates/proteins at 50 ng/Lane. Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500417) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells labeling KMT2D with Rabbit anti-KMT2D antibody (HA500417) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KMT2D antibody (HA500417) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.