LRRTM1 Rabbit Polyclonal Antibody
cat.: HA500418
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse LRRTM1 aa 320-360 / 522 (Extracellular). |
| Positive control: | SH-SY5Y cell lysate, N2A cell lysate, rat brain tissue lysate, N2A, SH-SY5Y. |
| Subcellular location: | Cell membrane, postsynaptic cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: Q86UE6 Human | Q8K377 Mouse |
| Alternative names: | 4632401D06Rik AW125451 FLJ32082 Leucine rich repeat transmembrane neuronal 1 Leucine rich repeat transmembrane neuronal 1 protein Leucine-rich repeat transmembrane neuronal protein 1 LRRT1_HUMAN LRRTM1 Lrrtm1 leucine rich repeat transmembrane neuronal 1 OTTHUMP00000194829 |
Images
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Fig1:
Western blot analysis of LRRTM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500418, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: N2A cell lysate Lane 1: Rat brain tissue lysate |
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Fig2: ICC staining of LRRTM1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500418, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of LRRTM1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500418, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.