DKK2 Rabbit Polyclonal Antibody
cat.: HA500420
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1.787ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DKK2 aa 70~259/259
Positive control: mouse skeletal muscle- tissue lysate, A549 cell lysates.
Subcellular location: Protein located outside the cell membrane(s).
Recommended Dilutions:
  WB

1:5000
Uniprot #: SwissProt: Q9UBU2 Human | Q9QYZ8 Mouse | D3ZN48 Rat
Alternative names: Anti-DKK2 DKK2 (N70) Antibody DKK2 Polyclonal Antibody DKK2 Monoclonal Antibody
Images
HA500420_1.jpg Fig1: Western blot analysis of DKK2 on different lysates with Rabbit anti-DKK2 antibody (HA500420) at 1/5,000 dilution.

Lane 1: A549 (Human lung adenocarcinoma cell)


Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500420, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 28 kDa
Observed band size: 28 kDa
HA500420_2.jpg Fig2: Western blot analysis of DKK2 on different lysates with Rabbit anti-DKK2 antibody (HA500420) at 1/5,000 dilution.

Lane 1: Mouse skeletal muscle tissue lysate

Lysates/proteins at 40 µg/Lane.
Exposure time: 3 minutes; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500420, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 28 kDa
Observed band size: 28 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.