| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.787ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DKK2 aa 70~259/259 |
| Positive control: | mouse skeletal muscle- tissue lysate, A549 cell lysates. |
| Subcellular location: | Protein located outside the cell membrane(s). |
| Recommended Dilutions:
WB |
1:5000 |
| Uniprot #: | SwissProt: Q9UBU2 Human | Q9QYZ8 Mouse | D3ZN48 Rat |
| Alternative names: | Anti-DKK2 DKK2 (N70) Antibody DKK2 Polyclonal Antibody DKK2 Monoclonal Antibody |
|
Fig1:
Western blot analysis of DKK2 on different lysates with Rabbit anti-DKK2 antibody (HA500420) at 1/5,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500420, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |
|
Fig2:
Western blot analysis of DKK2 on different lysates with Rabbit anti-DKK2 antibody (HA500420) at 1/5,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500420, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |