DKK2 Rabbit Polyclonal Antibody
cat.: HA500420
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.787ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DKK2 aa 70~259/259 |
| Positive control: | HL-60 cell lysate, mouse brain tissue lysate, A549 cell lysates. |
| Subcellular location: | Protein located outside the cell membrane(s). |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q9UBU2 Human | Q9QYZ8 Mouse | D3ZN48 Rat |
| Alternative names: | Anti-DKK2 DKK2 (N70) Antibody DKK2 Polyclonal Antibody DKK2 Monoclonal Antibody |
Images
|
Fig1:
Western blot analysis of DKK2 on different lysates with Rabbit anti-DKK2 antibody (HA500420) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12%/15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500420) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Rabbit IgG-HRP antibody (HA1001) at 1:300,000 dilution was used for 30 minutes at room temperature. |
|
Fig2:
Western blot analysis of DKK2 on A549 cell lysates with Rabbit anti-DKK2 antibody (HA500420) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28, 50, 65 kDa Exposure time: 2 minutes; 12%/15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500420) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Rabbit IgG-HRP antibody (HA1001) at 1:300,000 dilution was used for 30 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.