TAB3 Rabbit Polyclonal Antibody
cat.: HA500421
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.854ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TAB3 aa 513-712 / 712. |
| Positive control: | NIH/3T3 cell lysates, human striated muscle tissue. |
| Subcellular location: | Cytosol, extracellular exosome, plasma membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600 |
| Uniprot #: | SwissProt: Q8N5C8 Human | Q571K4 Mouse |
| Alternative names: | MAP3K7IP 3 Mitogen-activated protein kinase kinase kinase 7-interacting protein 3 NAP1 NF-kappa-B-activating protein 1 NFkB activating protein 1 TAB-3 Tab3 TAB3_HUMAN TAK1 binding protein 3 TAK1-binding protein 3 TGF-beta-activated kinase 1 and MAP3K7-binding protein 3 TGF-beta-activated kinase 1-binding protein 3 |
Images
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Fig1:
Western blot analysis of TAB3 on NIH/3T3 cell lysates with Rabbit anti-TAB3 antibody (HA500421) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 95 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500421) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-TAB3 antibody (HA500421) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500421) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.