Cathepsin S Rabbit Polyclonal Antibody
cat.: HA500422
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC(Intra) |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.305ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cathepsin S aa 101-331 / 331. |
| Positive control: | THP-1 (Human acute monoblastic leukemia cell) cell lysate, THP-1 treated with 80nM TPA for 24 hours then treated with 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate, THP-1, SiHa. |
| Subcellular location: | Lysosome, Secreted, Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
WB IF-Cell FC(Intra) |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P25774 Human | O70370 Mouse | Q02765 Rat |
| Alternative names: | Cathepsin S CathepsinS CATS_HUMAN Ctss |
Images
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Fig1:
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody (HA500422) at 1/5,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: THP-1 treated with 80nM TPA for 24 hours then treated with 100ng/mL LPS for 7 hours, add 1μg/mL BFA for last 3 hours cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500422, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37/25 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: THP-1 (Human acute monoblastic leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA500422, 1/100, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling Cathepsin S with Rabbit anti-Cathepsin S antibody (HA500422) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin S antibody (HA500422) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Application: Flow Cytometry (Intra) Species: Human Sample: THP-1 (Human acute monoblastic leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA500422 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.