Cathepsin S Rabbit Polyclonal Antibody
cat.: HA500422
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.305ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cathepsin S aa 101-331 / 331. |
| Positive control: | THP-1 cell lysate, Hela cell lysate, A431 cell lysate, PC-12 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, SiHa. |
| Subcellular location: | Lysosome, Secreted, Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P25774 Human | O70370 Mouse | Q02765 Rat |
| Alternative names: | Cathepsin S CathepsinS CATS_HUMAN Ctss |
Images
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Fig1:
Western blot analysis of Cathepsin S on different lysates with Rabbit anti-Cathepsin S antibody (HA500422) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: A431 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 25/35/40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500422) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SiHa cells labeling Cathepsin S with Rabbit anti-Cathepsin S antibody (HA500422) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin S antibody (HA500422) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.