PSMC5 Rabbit Polyclonal Antibody
cat.: HA500423
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.13ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSMC5 aa 207-406 / 406. |
| Positive control: | A431 cell lysate, MCF-7 cell lysate, Hela cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat brain tissue, human lung carcinoma tissue, A549, Hela. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P62195 Human | P62196 Mouse | P62198 Rat |
| Alternative names: | 26S protease regulatory subunit 8 26S proteasome AAA-ATPase subunit RPT6 Cim3 MSUG1 protein p45 p45/SUG Proteasome 26S ATPase subunit 5 Proteasome 26S subunit ATPase 5 Proteasome prosome macropain 26S subunit ATPase 5 Proteasome subunit p45 PRS8_HUMAN PSMC5 Rpt6 S8 SUG1 Tat binding protein homolog 10 TBP10 Thyroid hormone receptor interacting protein 1 Thyroid hormone receptor-interacting protein 1 Thyroid receptor interactor 1 TRIP1 TRIP1(SUG1) |
Images
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Fig1:
Western blot analysis of PSMC5 on different lysates with Rabbit anti-PSMC5 antibody (HA500423) at 1/1,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane) Lane 2: MCF-7 cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: HepG2 cell lysate (10 µg/Lane) Lane 5: Mouse testis tissue lysate (20 µg/Lane) Lane 6: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 46/70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMC5 antibody (HA500423) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500423) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PSMC5 antibody (HA500423) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500423) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of A549 cells labeling PSMC5 with Rabbit anti-PSMC5 antibody (HA500423) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMC5 antibody (HA500423) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of Hela cells labeling PSMC5 with Rabbit anti-PSMC5 antibody (HA500423) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMC5 antibody (HA500423) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.