RAC1/2/3 Rabbit Polyclonal Antibody
cat.: HA500424
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.958ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RAC3 aa 23-192 / 192. |
| Positive control: | Rat brain tissue lysate, human brain tissue lysate, rat skin tissue, SH-SY5Y, mouse brain tissue lysate, mouse cerebellum tissue lysate. |
| Subcellular location: | Cytoplasm, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:600 1:200 |
| Uniprot #: | SwissProt: P15153 Human | P60763 Human | P63000 Human | P60764 Mouse | P63001 Mouse | Q05144 Mouse Entrez Gene: 688319 Rat |
| Alternative names: | OTTMUSP00000004488 p21 Rac3 p21-Rac3 Rac1B RAC3 RAC3_HUMAN RAS related C3 botulinum substrate 3 Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) Ras-related C3 botulinum toxin substrate 3 Rho family small GTP binding protein Rac3 RP23-84C12.18 |
Images
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Fig1:
Western blot analysis of RAC1/2/3 on different lysates with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500424) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RAC1/2/3 on different lysates with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/500 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500424) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500424) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling RAC1/2/3 with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.