Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2.305ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 58 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human IFNAR2 aa 466-515 / 515. |
Positive control: | Hela cell lysate, SW480 cell lysate, LO2 cell lysate, rat liver tissue lysate. |
Subcellular location: | Cell membrane; Secreted. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: P48551 Human Entrez Gene: 686326 Rat |
Alternative names: | Human interferon alpha/beta receptor IFN alpha REC IFN R IFN-a/ßR2 IFN-a/ßRß IFN-alpha binding protein IFN-alpha/beta receptor 2 IFN-R-2 IFNABR IFNAR2 IFNARB IFNR INAR2_HUMAN Interferon (alpha beta and omega) receptor 2 Interferon alpha binding protein Interferon alpha/beta receptor 2 Interferon alpha/beta receptor beta chain Type I interferon receptor 2 Type I interferon receptor |
Fig1:
Western blot analysis of IFNAR2 on different lysates with Rabbit anti-IFNAR2 antibody (HA500425) at 1/500 dilution. Lane 1: Hela cell lysate (30 µg/Lane) Lane 2: SW480 cell lysate (10 µg/Lane) Lane 3: LO2 cell lysate (20 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 58 kDa Observed band size: 58/95/100 kDa (Predicted glycosylation modification) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500425) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |