IL3RA Rabbit Polyclonal Antibody
cat.: HA500426
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1.491ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human IL3RA aa 329-378 / 378.
Positive control: THP-1 cell lysate, Hela cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
1:500
Uniprot #: SwissProt: P26951 Human
Alternative names: CD123 CD123 antigen hIL 3Ra hIL-3Ra hIL3Ra IL 3 receptor alpha SP2 isoform IL 3R alpha IL-3 receptor subunit alpha IL-3R subunit alpha IL-3R-alpha IL-3RA IL3 Receptor alpha IL3R Il3ra IL3RA_HUMAN IL3RAX IL3RAY IL3RX IL3RY Interleukin 3 receptor alpha chain Interleukin 3 receptor, alpha (low affinity) Interleukin 3 receptor, alpha Interleukin-3 Receptor alpha Interleukin-3 receptor subunit alpha Interleukin3 receptor Interleukin3 receptor, Y-chromosomal
Images
HA500426_1.jpg Fig1: Western blot analysis of IL3RA on different lysates with Rabbit anti-IL3RA antibody (HA500426) at 1/500 dilution.

Lane 1: THP-1 cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 70 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500426) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.