| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.491ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IL3RA aa 329-378 / 378. |
| Positive control: | THP-1 cell lysate |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P26951 Human |
| Alternative names: | CD123 CD123 antigen hIL 3Ra hIL-3Ra hIL3Ra IL 3 receptor alpha SP2 isoform IL 3R alpha IL-3 receptor subunit alpha IL-3R subunit alpha IL-3R-alpha IL-3RA IL3 Receptor alpha IL3R Il3ra IL3RA_HUMAN IL3RAX IL3RAY IL3RX IL3RY Interleukin 3 receptor alpha chain Interleukin 3 receptor, alpha (low affinity) Interleukin 3 receptor, alpha Interleukin-3 Receptor alpha Interleukin-3 receptor subunit alpha Interleukin3 receptor Interleukin3 receptor, Y-chromosomal |
|
Fig1:
Western blot analysis of IL3RA / CD123 on THP-1 cell lysate with Rabbit anti-IL3RA / CD123 antibody (HA500426) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500426, 1/500 5% NFDM/TBST, room temperature for 2 hours Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1:300,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 70 kDa |