SKP2 Rabbit Polyclonal Antibody
cat.: HA500427
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.783ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46/48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SKP2 aa 1-150 / 424. |
| Positive control: | Siha cell lysate, HepG2 cell lysate, 293T cell lysate, HeLa, human placenta tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:400 1:100 |
| Uniprot #: | SwissProt: Q13309 Human |
| Alternative names: | CDK2/Cyclin A associated protein p45 Cyclin A/CDK2 associated protein p45 Cyclin-A/CDK2-associated protein p45 F box protein Skp2 F box/LRR repeat protein 1 F-box protein Skp2 F-box/LRR-repeat protein 1 FBL 1 FBL1 FBXL 1 FBXL1 FLB 1 FLB1 MGC1366 p45 p45skp2 S phase kinase associated protein 2 (p45) S phase kinase associated protein 2 S-phase kinase-associated protein 2 S-phase kinase-associated protein 2 E3 ubiquitin protein ligase SKP 2 Skp2 SKP2_HUMAN |
Images
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Fig1:
Western blot analysis of SKP2 on different lysates with Rabbit anti-SKP2 antibody (HA500427) at 1/1,000 dilution. Lane 1: Siha cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Predicted band size: 46/48 kDa Observed band size: 46/48 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500427) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling SKP2 with Rabbit anti-SKP2 antibody (HA500427) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SKP2 antibody (HA500427) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-SKP2 antibody (HA500427) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500427) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.