STAM1 Rabbit Polyclonal Antibody
cat.: HA500430
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human 30-55aa |
| Positive control: | Siha, SHSY5Y, human kidney tissue, human colon tissue, Rat testis tissue. |
| Subcellular location: | Cytoplasm; Early endosome membrane |
| Recommended Dilutions:
IF-Cell IHC-P |
1:200 1:200-1:600 |
| Uniprot #: | SwissProt: Q92783 Human | A0A0G2JTT5 Rat |
| Alternative names: | DKFZp686J2352 HSE1 homolog OTTHUMP00000019237 Signal transducing adapter molecule 1 signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 STAM 1 STAM STAM-1 STAM1_HUMAN |
Images
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Fig1:
Immunocytochemistry analysis of Siha cells labeling STAM1 with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling STAM1 with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAM1 antibody (HA500430) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500430) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-STAM1 antibody (HA500430) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500430) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded Rat testis tissue with Rabbit anti-STAM1 antibody (HA500430) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500430) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.