TRRAP Rabbit Polyclonal Antibody
cat.: HA500431
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.6795ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 438kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRRAP aa 2,601-2,910 / 3,859. |
| Positive control: | K-562 cell lysate, 293T cell lysate, PC-12 cell lysate, L6 cell lysate, PMVEC cell lysate, rat brain tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: Q9Y4A5 Human | Q80YV3 Mouse | A0A8I5Y6J8 Rat |
| Alternative names: | 350/400 kDa PCAF associated factor PAF 350/400 PAF 400 PAF350/400 PAF400 STAF 40 STAF40 TR AP Tra 1 Tra1 Tra1 homolog Transactivation / transformation domain associated protein Transactivation/transformation domain associated protein Transformation/transcription domain associated protein TRAP |
Images
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Fig1:
Western blot analysis of TRRAP on different lysates with Rabbit anti-TRRAP antibody (HA500431) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: 293T cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 438 kDa Observed band size: 438 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500431) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TRRAP on different lysates with Rabbit anti-TRRAP antibody (HA500431) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 2: L6 cell lysate Lane 3: PMVEC cell lysate Predicted band size: 438 kDa Observed band size: 438 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500431) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TRRAP antibody (HA500431) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500431) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.