Glypican 4 Rabbit Polyclonal Antibody
cat.: HA500432
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Glypican 4 450-465aa |
| Positive control: | Mouse pancreas tissue lysate, rat pancreas tissue lysate, Hela. |
| Subcellular location: | Cell membrane, Secreted, extracellular space |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: O75487 Human | P51655 Mouse | Q642B0 Rat |
| Alternative names: | dJ900E8.1 glypican 4 Glypican proteoglycan 4 Gpc4 GPC4_HUMAN K Glypican K-glypican Kglypican OTTHUMP00000024052 Secreted glypican 4 Secreted glypican-4 UNQ474/PRO937 |
Images
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Fig1:
Western blot analysis of Glypican 4 on different lysates with Rabbit anti-Glypican 4 antibody (HA500432) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate (40 µg/Lane) Lane 2: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500432) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Hela cells labeling Glypican 4 with Rabbit anti-Glypican 4 antibody (HA500432) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Glypican 4 antibody (HA500432) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.