Frizzled 7 Rabbit Polyclonal Antibody
cat.: HA500433
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Frizzled 7 aa 525-574 / 574. |
| Positive control: | HepG2 cell lysate, 293 cell lysate, 293T cell lysate, human skeletal muscle tissue lysate. |
| Subcellular location: | Cell membrane, Endosome membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: O75084 Human |
| Alternative names: | Frizzled 7, seven transmembrane spanning receptor frizzled class receptor 7 Frizzled drosophila homolog of 7 Frizzled family receptor 7 Frizzled homolog 7 Frizzled-7 Frizzled7 Fz 7 Fz-7 Fz7 FZD 7 FZD7 FZD7_HUMAN FzE 3 FzE3 hFz 7 hFz7 Homolog of Drosophila Frizzled 7 OTTHUMP00000163742 |
Images
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Fig1:
Western blot analysis of Frizzled 7 on different lysates with Rabbit anti-Frizzled 7 antibody (HA500433) at 1/500 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Human skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500433) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.