Os11g0569800 protein Rabbit Polyclonal Antibody
cat.: HA500434
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rice |
| Applications: | IF-Tissue, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 86 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rice Os11g0569800 protein aa 450-550 / 791. |
| Positive control: | Rice tissue. |
| Subcellular location: | Integral component of membrane. |
| Recommended Dilutions:
IF-Tissue IHC-P |
1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q2R2D4 Rice |
| Alternative names: | Receptor kinase, putative, expressed LOC_Os11g36190 Os11g0569800 |
Images
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Fig1: Immunofluorescence staining of paraffin- embedded rice using anti-Os11g0569800 protein rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with HA500434 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Os11g0569800 protein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500434, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.