TRPM2 Rabbit Polyclonal Antibody
cat.: HA500437
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 172 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse TRPM2 aa 100-150 / 1503 (Cytoplasmic). |
| Positive control: | THP-1 cell lysate, PC-3 cell lysate, rat cerebellum tissue, human bone tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Lysosome, cell membrane, perikaryon, cell projection, cytoplasmic vesicle. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: O94759 Human | Q91YD4 Mouse | Q5G856 Rat |
| Alternative names: | EREG 1 EREG1 Estrogen responsive element associated gene 1 Estrogen responsive element associated gene 1 protein Estrogen-responsive element-associated gene 1 protein KNP 3 KNP3 Long transient receptor potential channel 2 LTRPC 2 LTrpC-2 LTrpC2 MGC133383 NUDT9H NUDT9L1 putative Ca2+ channel protein putative Ca2+ channel protein Transient receptor potential cation channel subfamily M member 2 Transient receptor potential channel 7 TRPC 7 TrpC7 TRPM 2 Trpm2 TRPM2_HUMAN |
Images
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Fig1:
Western blot analysis of TRPM2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500437, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: PC-3 cell lysate Predicted band size: 171 kDa Observed band size: 120 kDa (N terminal) |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-TRPM2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500437, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human bone tissue using anti-TRPM2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500437, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TRPM2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500437, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of TRPM2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500437, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.