TRPM2 Rabbit Polyclonal Antibody
cat.: HA500437
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 171 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse TRPM2 aa 100-150 / 1503 (Cytoplasmic). |
| Positive control: | THP-1 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, SH-SY5Y. |
| Subcellular location: | Lysosome, cell membrane, perikaryon, cell projection, cytoplasmic vesicle. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: O94759 Human | Q91YD4 Mouse | Q5G856 Rat |
| Alternative names: | EREG 1 EREG1 Estrogen responsive element associated gene 1 Estrogen responsive element associated gene 1 protein Estrogen-responsive element-associated gene 1 protein KNP 3 KNP3 Long transient receptor potential channel 2 LTRPC 2 LTrpC-2 LTrpC2 MGC133383 NUDT9H NUDT9L1 putative Ca2+ channel protein putative Ca2+ channel protein Transient receptor potential cation channel subfamily M member 2 Transient receptor potential channel 7 TRPC 7 TrpC7 TRPM 2 Trpm2 TRPM2_HUMAN |
Images
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Fig1:
Western blot analysis of TRPM2 on different lysates with Rabbit anti-TRPM2 antibody (HA500437) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HepG2 cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 171 kDa Observed band size: 171/120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of TRPM2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500437, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.