IL-6R Rabbit Polyclonal Antibody
cat.: HA500438
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-6R aa 20-69/468. |
| Positive control: | Daudi cell lysate, A549 cell lysate, K562 cell lysate, Hela, human fetal skeletal muscle tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:200 1:600 |
| Uniprot #: | SwissProt: P08887 Human |
| Alternative names: | CD 126 CD126 CD126 antigen gp80 IL 6R 1 IL 6R alpha IL 6R IL-6 receptor alpha chain IL-6 receptor subunit alpha IL-6R 1 IL-6R subunit alpha IL-6R-alpha IL-6RA IL6Q Il6r IL6RA IL6RA_HUMAN IL6RQ Interleukin 6 receptor interleukin 6 receptor, alpha Interleukin-6 receptor subunit alpha Membrane glycoprotein 80 MGC30256 |
Images
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Fig1:
Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA500438) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: A549 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 47 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500438) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of IL-6R in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500438, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-IL-6R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500438, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.