OSMR Rabbit Polyclonal Antibody
cat.: HA500439
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 111 kDa
Isotype: IgG
Immunogen: Recombinant protein within human OSMR aa 1-740.
Positive control: HeLa cell lysate, A375 cell lysate, Human fetal skeletal muscle tissue, Human kidney tissue, Human liver tissue, Hela, PC-3M, Siha.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:2,000
1:200-1:600
1:200
Uniprot #: SwissProt: Q99650 Human
Alternative names: IL-31 receptor subunit beta IL-31R subunit beta IL-31R-beta IL-31RB Interleukin-31 receptor subunit beta MGC140467 MGC150626 MGC150627 MGC75127 Oncostatin M receptor Oncostatin M specific receptor subunit beta Oncostatin M-specific receptor, beta Oncostatin-M-specific receptor subunit beta Osmr OSMR_HUMAN OSMRB
Images
HA500439_1.jpg Fig1: Western blot analysis of OSMR on different lysates with Rabbit anti-OSMR antibody (HA500439) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A375 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 111 kDa
Observed band size: 180 kDa

Exposure time: 1 minute 23 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500439) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500439_2.png Fig2: Immunohistochemical analysis of paraffin-embedded Human fetal skeletal muscle tissue with Rabbit anti-OSMR antibody (HA500439) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500439) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500439_3.png Fig3: Immunohistochemical analysis of paraffin-embedded Human kidney tissue with Rabbit anti-OSMR antibody (HA500439) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500439) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500439_4.png Fig4: Immunohistochemical analysis of paraffin-embedded Human liver tissue with Rabbit anti-OSMR antibody (HA500439) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500439) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500439_5.png Fig5: Immunocytochemistry analysis of Hela cells labeling OSMR with Rabbit anti-OSMR antibody (HA500439) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.05% Triton X-100 in PBS for 15 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OSMR antibody (HA500439) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA500439_6.png Fig6: Immunocytochemistry analysis of PC-3M cells labeling OSMR with Rabbit anti-OSMR antibody (HA500439) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.05% Triton X-100 in PBS for 15 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OSMR antibody (HA500439) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA500439_7.png Fig7: Immunocytochemistry analysis of Siha cells labeling OSMR with Rabbit anti-OSMR antibody (HA500439) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.05% Triton X-100 in PBS for 15 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OSMR antibody (HA500439) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.