STAM2 Rabbit Polyclonal Antibody
cat.: HA500440
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.9865ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human STAM2 450-480 aa |
| Positive control: | SK-OV-3 cell lysate, SK-Br-3 cell lysate, MCF-7 cell lysate, HepG2 cell lysate, Jurkat cell lysate, THP-1 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, Siha, PC-3. |
| Subcellular location: | Cytoplasm, Early endosome membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:200 1ug/ml |
| Uniprot #: | SwissProt: O75886 Human | O88811 Mouse | Q5XHY7 Rat |
| Alternative names: | DKFZp564C047 Hbp Hrs-binding protein HSE1 homolog Signal transducing adapter molecule 2 Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 Signal transducing adaptor molecule 2A Signal transducing adaptor molecule 2B Signal transducing adaptor molecule STAM like protein containing SH3 and ITAM domains 2 STAM-2 Stam2 STAM2_HUMAN STAM2A STAM2B |
Images
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Fig1:
Western blot analysis of STAM2 on different lysates with Rabbit anti-STAM2 antibody (HA500440) at 1/1,000 dilution. Lane 1: SK-OV-3 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF-7 cell lysate Lane 4: HepG2 cell lysate Lane 5: Jurkat cell lysate Lane 6: THP-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Siha cells labeling STAM2 with Rabbit anti-STAM2 antibody (HA500440) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-STAM2 antibody (HA500440) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of PC-3 cells labeling STAM2 with Rabbit anti-STAM2 antibody (HA500440) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-STAM2 antibody (HA500440) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling STAM2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500440, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.