PSMD13 Rabbit Polyclonal Antibody
cat.: HA500441
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSMD13 1-160aa |
| Positive control: | HepG2 cell lysate, Hela cell lysate, Siha cell lysate, SH-SY5Y cell lysate, NIH3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, Siha, HepG2, Hela. |
| Subcellular location: | cytosol, extracellular region, nucleoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:2,000 1:200 |
| Uniprot #: | SwissProt: Q9UNM6 Human | Q9WVJ2 Mouse | B0BN93 Rat |
| Alternative names: | 26S proteasome non ATPase regulatory subunit 13 26S proteasome non-ATPase regulatory subunit 13 26S proteasome regulatory subunit p40.5 26S proteasome regulatory subunit RPN9 26S proteasome regulatory subunit S11 26S proteasome subunit p40.5 HSPC027 P40.5 Proteasome (prosome, macropain) 26S subunit, non ATPase, 13 Proteasome 26S subunit, non-ATPase 13 PSD13_HUMAN psmD13 Rpn9 S11 |
Images
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Fig1:
Western blot analysis of PSMD13 on different lysates with Rabbit anti-PSMD13 antibody (HA500441) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Hela cell lysate (20 µg/Lane) Lane 3: Siha cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: NIH3T3 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Lane 8: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500441) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Siha cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of HepG2 cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunocytochemistry analysis of Hela cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.