Inversin Rabbit Polyclonal Antibody
cat.: HA500442
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 118 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Inversin aa 901-1,050 / 1,065. |
| Positive control: | HepG2 cell lysate, K562 cell lysate, 293T cell lysate, 293 cell lysate, rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Y283 Human | O89019 Mouse Entrez Gene: 313228 Rat |
| Alternative names: | INV Inversion of embryo turning homolog inversion of embryonic turning INVS Nephrocystin 2 Nephrocystin-2 Nephrocystin2 nephronophthisis 2 (infantile) NPH2 NPHP2 |
Images
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Fig1:
Western blot analysis of Inversin on different lysates with Rabbit anti-Inversin antibody (HA500442) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: K562 cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: 293 cell lysate (10 µg/Lane) Lane 5: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 118 kDa Observed band size: 95 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500442) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.