PEPT1 Rabbit Polyclonal Antibody
cat.: HA500444
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Chicken, Dog, Pig, Cow |
| Applications: | WB, ELISA, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 79 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PEPT1: 101-200/714(chk) <Cytoplasmic> |
| Positive control: | SH-Sy5Y. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB ELISA FC |
1:500-1:2,000 1:5,000-1:10,000 2μg/Test |
| Uniprot #: | SwissProt: P46059 Human |
| Alternative names: | +peptide cotransporter HPECT1 HPEPT1 Human peptide transporter (HPEPT1) mRNA complete cds Intestinal H Intestinal H(+)/peptide cotransporter Oligopeptide transporter Oligopeptide transporter small intestine isoform PEPT1 Peptide transporter 1 S15A1_HUMAN Slc15a1 small intestine isoform Solute carrier family 15 (oligopeptide transporter) member 1 Solute carrier family 15 member 1 |
Images
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Fig1:
Black line : Positive blank control SH-Sy5Y); Negative blank control (A431） Green line : Primary Antibody (Rabbit Anti-PEPT1 antibody (HA500444) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488) SH-Sy5Y（Positive）and A431（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEPT1 Antibody(HA500444)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
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Fig2:
Blank control: HCCLM3(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.